资料介绍
Abstract
Rapid and accurate methods are needed to quickly detect mycotoxigenic
fungi such as those in the genera of Aspergillus, Penicillium, and Fusarium.
This study was conducted to develop and test a multiplex real-time
qPCR assay to detect and quantify these agriculturally and economically
important fungi. PCR primers were designed to amplify the internal
transcribed spacer (ITS) regions of rDNA from three mycotoxigenic
genera. Genus-specific probes were designed from the ITS sequences of
the most important mycotoxigenic species of Fusarium, Penicillium, and
Aspergillus. Fluorophores were attached to the 5’ end of the probes, which
were detectable with the Stratagene Mx3000P QPCR system. The linear
detection range of the multiplex assay was between 10 pg to 10 ng of
DNA. This assay could be used as an initial step to evaluate the potential
for mycotoxin accumulation in various agricultural commodities.
Rapid and accurate methods are needed to quickly detect mycotoxigenic
fungi such as those in the genera of Aspergillus, Penicillium, and Fusarium.
This study was conducted to develop and test a multiplex real-time
qPCR assay to detect and quantify these agriculturally and economically
important fungi. PCR primers were designed to amplify the internal
transcribed spacer (ITS) regions of rDNA from three mycotoxigenic
genera. Genus-specific probes were designed from the ITS sequences of
the most important mycotoxigenic species of Fusarium, Penicillium, and
Aspergillus. Fluorophores were attached to the 5’ end of the probes, which
were detectable with the Stratagene Mx3000P QPCR system. The linear
detection range of the multiplex assay was between 10 pg to 10 ng of
DNA. This assay could be used as an initial step to evaluate the potential
for mycotoxin accumulation in various agricultural commodities.
下载该资料的人也在下载
下载该资料的人还在阅读
更多 >
- 被动元器件行业报告 6次下载
- AI遇冷?2023从融资再看AI“芯”赛道? 0次下载
- 2023年人工智能产业概况及应用趋势分析 0次下载
- EDA市场现状分析及趋势 3次下载
- 第三代半导体GaN产业链研究 1次下载
- 解密2021年华为小米投资迅猛增长的背后逻辑 1次下载
- 国际形势突变,中国芯片公司 能否“风景这边独好” 1次下载
- AR/VR市场情况及上游产业链分析 1次下载
- 高效高功率同步整流升压DC-DC芯片AMT6802 19次下载
- 华为统一通信与协作市场发展趋势白皮书下载 1次下载
- 【Vivado那些事】Vivado下怎么查看各子模块的资源占用? 10次下载
- 多层芯片应用中的封装挑战和解决方案 20次下载
- Agilent and Stratagene Protei 24次下载
- Stratagene Mutagenesis Solutio 18次下载
- 介绍如何利用XPLA3的通用控制项的优势 15次下载
- 关于AI遗传算法的详解 8.3w次阅读
- USB23年发展史,多了什么变化? 8.2w次阅读
- 分析总结网优的过去及现状、未来发展趋势 2589次阅读
- 新规划PCI核查工具的使用方法和应用事例 2435次阅读
- 5G时代到来,各个国家的2G/3G也将开始“退休” 662次阅读
- 宽禁带器件和仿真环境介绍 1317次阅读
- 浅谈泰克示波器鲜为人知的10个特异功能 3675次阅读
- 浅谈差分处理和信号分析 1742次阅读
- 怎么使得电源开关并联还能均流 3460次阅读
- 在贴片加工厂中有哪些安全防护需要了解 1197次阅读
- 复合放大器实现高精度的高输出驱动能力 获得最佳的性能 1468次阅读
- 用降压型稳压器或线性稳压器电源时值来会为负载供电 872次阅读
- 锂电池并联充电时保护板均衡原理 2.7w次阅读
- 更小更智能的电机控制器推进HEV/EV市场 1023次阅读
- 三个命令生成的报告中均可显示Clock Skew的具体数值 4338次阅读
下载排行
本周
- 1电子电路原理第七版PDF电子教材免费下载
- 0.00 MB | 1491次下载 | 免费
- 2单片机典型实例介绍
- 18.19 MB | 95次下载 | 1 积分
- 3S7-200PLC编程实例详细资料
- 1.17 MB | 27次下载 | 1 积分
- 4笔记本电脑主板的元件识别和讲解说明
- 4.28 MB | 18次下载 | 4 积分
- 5开关电源原理及各功能电路详解
- 0.38 MB | 11次下载 | 免费
- 6100W短波放大电路图
- 0.05 MB | 4次下载 | 3 积分
- 7基于单片机和 SG3525的程控开关电源设计
- 0.23 MB | 4次下载 | 免费
- 8基于AT89C2051/4051单片机编程器的实验
- 0.11 MB | 4次下载 | 免费
本月
- 1OrCAD10.5下载OrCAD10.5中文版软件
- 0.00 MB | 234313次下载 | 免费
- 2PADS 9.0 2009最新版 -下载
- 0.00 MB | 66304次下载 | 免费
- 3protel99下载protel99软件下载(中文版)
- 0.00 MB | 51209次下载 | 免费
- 4LabView 8.0 专业版下载 (3CD完整版)
- 0.00 MB | 51043次下载 | 免费
- 5555集成电路应用800例(新编版)
- 0.00 MB | 33562次下载 | 免费
- 6接口电路图大全
- 未知 | 30320次下载 | 免费
- 7Multisim 10下载Multisim 10 中文版
- 0.00 MB | 28588次下载 | 免费
- 8开关电源设计实例指南
- 未知 | 21539次下载 | 免费
总榜
- 1matlab软件下载入口
- 未知 | 935053次下载 | 免费
- 2protel99se软件下载(可英文版转中文版)
- 78.1 MB | 537793次下载 | 免费
- 3MATLAB 7.1 下载 (含软件介绍)
- 未知 | 420026次下载 | 免费
- 4OrCAD10.5下载OrCAD10.5中文版软件
- 0.00 MB | 234313次下载 | 免费
- 5Altium DXP2002下载入口
- 未知 | 233046次下载 | 免费
- 6电路仿真软件multisim 10.0免费下载
- 340992 | 191183次下载 | 免费
- 7十天学会AVR单片机与C语言视频教程 下载
- 158M | 183277次下载 | 免费
- 8proe5.0野火版下载(中文版免费下载)
- 未知 | 138039次下载 | 免费
评论
查看更多